-
March 15th, 2013, 06:52 PM #1IVF Advice Coach
- Join Date
- Dec 2010
- Location
- The Internet
- Posts
- 24,567
- Post Thanks / Like
- Blog Entries
- 6
- Downloads
- 0
- Uploads
- 0
What Stage is best(if any) for freezing an embryo
Hi Dr Wegner.
I am asking for our members-
Many of us are opting for day 5 biopsies and embryos generally need to be blastocysts in order to have a day 5 biopsy. Do you think it matters what stage an embryo is frozen in regards to surviving a thaw?
Meaning, early blast versus expanded, hatching or fully hatched- is there a reason to want to freeze before an embryo completely hatches from its shell or is any blast stage fine for freeze(assuming vitrification is used).
Thank you!Mom to
and my IVF/PGD
It's better to look back on life and say: "I can't believe I did that" than to look back and say "I wish I did that".
New to IVF/PGD for Family Balancing? Read this- Understanding IVF/PGD- a HT Guide for those New to the IVF/PGD Process
Need a Natural Swaying Plan? Naturally sway for a boy or a girl- Personalized Swaying Plans
Become a Dream Member to access the private forums
-
March 15th, 2013, 08:34 PM #2
Dear NuthinbutPink,
I wrote a post on this topic previously Fertility Lab Insider - Lessons learned from over fifteen years of working inside fertility labs. » Blog Archive » New Series: Q From U: biopsy where I looked at biopsy on day 5 vs day 6 and whether it is a good idea to wait for spontaneous hatching prior to biopsy.
Vitrification does very well at any stage. The only caveat is that for fully expanded blastocysts (straining at the shell but not yet breached), it is important to artificially collapse the blastocyst using either a laser or mechanical means. Collapsing the blastocyst allows full penetration of the cryopreservative solutions. If the blastocyst is early stage (not fully expanded) or has collapsed on its own or has hatched (even slightly) the solutions are able to penetrate just fine, so not extra intervention to collapse is needed.
In any group of embryos, there will likely be some at each blast stage so the technologist should be able to collapse the blast when needed prior to vitrification--or alternatively pull an embryo part way out of the shell to grab a piece for biopsy if it isn't spontaneously hatching in the timeframe for PGD. Hope this helps! Carole
-
March 15th, 2013, 09:00 PM #3IVF Advice Coach
- Join Date
- Dec 2010
- Location
- The Internet
- Posts
- 24,567
- Post Thanks / Like
- Blog Entries
- 6
- Downloads
- 0
- Uploads
- 0
So a fully hatched blast should freeze and thaw fine? There's no risk when its hatched from the shell completely?
Mom to
and my IVF/PGD
It's better to look back on life and say: "I can't believe I did that" than to look back and say "I wish I did that".
New to IVF/PGD for Family Balancing? Read this- Understanding IVF/PGD- a HT Guide for those New to the IVF/PGD Process
Need a Natural Swaying Plan? Naturally sway for a boy or a girl- Personalized Swaying Plans
Become a Dream Member to access the private forums
-
March 15th, 2013, 09:08 PM #4IVF Advice Coach
- Join Date
- Dec 2010
- Location
- The Internet
- Posts
- 24,567
- Post Thanks / Like
- Blog Entries
- 6
- Downloads
- 0
- Uploads
- 0
That is a great post! I missed that one. SART in Thailand is affiliated with Genea(Sidney IVF). GS is illegal in Australia but they can go to Thailand for treatment. They require hatching blasts in order to do a biopsy. That's their policy. Based on your post, I suspect people will be cautious when choosing a clinic that is that strict for biopsy.
Mom to
and my IVF/PGD
It's better to look back on life and say: "I can't believe I did that" than to look back and say "I wish I did that".
New to IVF/PGD for Family Balancing? Read this- Understanding IVF/PGD- a HT Guide for those New to the IVF/PGD Process
Need a Natural Swaying Plan? Naturally sway for a boy or a girl- Personalized Swaying Plans
Become a Dream Member to access the private forums
-
March 15th, 2013, 10:13 PM #5
-
May 24th, 2013, 04:16 AM #6
So glad I have read this great post, it's really helped with my decision process on what to do next!
Cycle 1 - SART (Feb '13): 10 retrieved, 10 fertilised, 5 to 5 day biopsy - 2XY, 1XX (biopsied twice)
Cycle 1 - FET (Mar '13): thawed with 50% cell degeneration BFN
Cycle 2 - SART (May '13): 13 retrieved, 7 fertilised, 2 to 5 day biopsy - 1 normal XY, 1 abnormal XX
Cycle 3 - HRC (Sept '13): 16 retrieved, 7 fertilised, 7 to day 5 biopsy - 1 Normal XY and 1 Normal XX - Transferred BFP!!!!!
Similar Threads
-
Freezing!!!
By Sunflower3 in forum Pregnancy TalkReplies: 8Last Post: November 30th, 2012, 01:21 PM -
TTC a boy - planning stage
By GerryO in forum IntroductionsReplies: 3Last Post: October 18th, 2012, 04:44 PM
So happy for you Treens, congratulations Sent from my SM-A225F using Tapatalk
Healthy baby girl :)